The PPM1F Polyclonal Antibody (CAB15363) is a valuable tool for researchers studying PPM1F, a phosphatase protein known for its role in cell cycle regulation and DNA damage response. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blotting applications. It binds to the PPM1F protein, allowing for accurate detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and cancer research.
PPM1F plays a crucial role in maintaining genomic stability and controlling cell division by dephosphorylating key proteins involved in these processes. Dysregulation of PPM1F has been linked to cancer development and progression, making it a promising target for therapeutic interventions. Understanding the function and regulation of PPM1F is essential for unlocking its potential as a biomarker and drug target in cancer therapy.
Product Name:
PPM1F Rabbit Polyclonal Antibody
SKU:
CAB15363
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1).
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
Purification Method:
Affinity purification
Gene ID:
9647
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPM1F Rabbit pAb (CAB15363) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
