The PPM1A Polyclonal Antibody (CAB6699) is a valuable tool for researchers studying PPM1A, a critical regulator of multiple cellular processes, including cell cycle progression, DNA damage response, and stress signaling. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By binding to the PPM1A protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in cell biology, cancer research, and drug development.PPM1A, also known as protein phosphatase Mg2+/Mn2+ dependent 1A, is involved in dephosphorylation events that control key signaling pathways crucial for cellular function and survival.
Dysregulation of PPM1A has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. Therefore, understanding the role of PPM1A in these pathological conditions is essential for developing targeted therapies and diagnostic tools.By utilizing the PPM1A Polyclonal Antibody (CAB6699), researchers can uncover the intricate mechanisms underlying PPM1A function and its impact on cellular physiology, paving the way for innovative advancements in biomedical research and personalized medicine.
Product Name:
PPM1A Rabbit Polyclonal Antibody
SKU:
CAB6699
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPM1A (NP_005029.1).
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5494
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PPM1A antibody (CAB6699) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
