The PPIF Polyclonal Antibody (CAB3208) is a valuable tool for researchers studying the protein PPIF, also known as cyclophilin D, which plays a key role in the regulation of mitochondrial functions and cell death pathways. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PPIF is a peptidyl-prolyl cis-trans isomerase that is involved in the regulation of mitochondrial permeability transition pore (MPTP), a critical component in the induction of cell death.
Dysregulation of PPIF has been implicated in a variety of diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer.By using the PPIF Polyclonal Antibody, researchers can detect and analyze PPIF expression in various cell types, providing insights into the role of this protein in disease pathogenesis. This antibody is an essential tool for studies in cell biology, mitochondrial dysfunction, and cell death mechanisms, offering new opportunities for therapeutic interventions targeting PPIF in various diseases.
Product Name:
PPIF Rabbit Polyclonal Antibody
SKU:
CAB3208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-207 of human PPIF (NP_005720.1).
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Purification Method:
Affinity purification
Gene ID:
10105
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PPIF Rabbit pAb (CAB3208) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
