The PPIE Polyclonal Antibody (CAB7061) is a powerful tool for researchers studying the PPIE protein, a key player in cellular processes such as protein folding and trafficking. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications.PPIE, also known as the peptidyl-prolyl cis-trans isomerase E, is essential for the proper folding of proteins and the regulation of protein function. Dysregulation of PPIE has been linked to various diseases, including cancer and neurodegenerative disorders.
By utilizing this antibody, researchers can gain valuable insights into the role of PPIE in these pathological conditions and potentially identify new therapeutic targets.With its ability to bind specifically to the PPIE protein, the CAB7061 antibody enables precise detection and analysis of PPIE expression in a variety of cell types. This makes it an invaluable tool for immunology and cancer research, offering researchers a deeper understanding of the molecular mechanisms underlying disease progression and potential treatment strategies.
Product Name:
PPIE Rabbit Polyclonal Antibody
SKU:
CAB7061
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CYP33; CYP-33; PPIE
Positive Sample:
HeLa,THP-1,Jurkat,HL-60,Mouse testis,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
Purification Method:
Affinity purification
Gene ID:
10450
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPIE Rabbit pAb (CAB7061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
