The PPAT Polyclonal Antibody (CAB6698) is specifically designed for research involving PPAT, an essential enzyme involved in the biosynthesis of purine nucleotides. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.PPAT, also known as phosphoribosyl pyrophosphate amidotransferase, plays a critical role in the de novo purine biosynthesis pathway, making it a key target for studies in biochemistry and molecular biology. Understanding the function and regulation of PPAT is essential for unraveling the mechanisms underlying purine metabolism and its implications in diseases such as cancer, metabolic disorders, and neurological conditions.
Researchers interested in investigating the role of PPAT in cellular metabolism and disease pathogenesis will find the PPAT Polyclonal Antibody (CAB6698) to be a valuable tool for their research endeavors. Its high specificity and sensitivity make it an ideal choice for studying PPAT expression and localization in different cell types and tissues, paving the way for new discoveries in the field of purine metabolism research.
Product Name:
PPAT Rabbit Polyclonal Antibody
SKU:
CAB6698
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
Purification Method:
Affinity purification
Gene ID:
5471
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of Rat spleen, using PPAT Rabbit pAb (CAB6698) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
