The PPAN Polyclonal Antibody (CAB7377) is a key tool for researchers studying the PPAN protein, which is involved in various cellular processes such as mitochondrial function and DNA replication. This antibody is raised in rabbits and has been validated for use in Western blot applications, ensuring high specificity and sensitivity in detecting PPAN protein levels in human samples.PPAN, also known as PAN2, is a vital component of the PAN complex, which plays a crucial role in RNA processing and mitochondrial RNA stability.
Dysregulation of PPAN has been linked to diseases like cancer and neurological disorders, making it a promising target for therapeutic interventions. By using the PPAN Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of PPAN in different cell types, advancing our understanding of its role in disease pathology.
Product Name:
PPAN Rabbit Polyclonal Antibody
SKU:
CAB7377
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).
The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.
Purification Method:
Affinity purification
Gene ID:
56342
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PPAN Rabbit pAb (CAB7377) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
