The PPA1 Polyclonal Antibody (CAB14050) is a valuable tool for researchers studying PPA1, a protein involved in cellular metabolism and energy production. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By targeting the PPA1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and metabolic research.PPA1, also known as pyrophosphatase 1, plays a crucial role in maintaining cellular energy balance by catalyzing the hydrolysis of inorganic pyrophosphate.
Dysregulation of PPA1 activity has been linked to metabolic disorders and diseases such as diabetes and cancer, making it a promising target for therapeutic interventions. Understanding the function of PPA1 is essential for uncovering its role in metabolic pathways and developing new treatment strategies for related conditions.
Product Name:
PPA1 Rabbit Polyclonal Antibody
SKU:
CAB14050
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1).
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Purification Method:
Affinity purification
Gene ID:
5464
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PPA1 antibody (CAB14050) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
