The PP2A B55 (PR55) (PPP2R2A) Monoclonal Antibody is a valuable tool for research involving the protein phosphatase 2A (PP2A) regulatory subunit B55, also known as PR55 and PPP2R2A. This antibody, generated in mice, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. It targets the PP2A B55 subunit, enabling precise detection and analysis in a variety of cell types.PP2A is a major serine/threonine phosphatase that regulates various cellular processes, including cell cycle progression, apoptosis, and signal transduction. The B55 regulatory subunit is involved in controlling PP2A activity and substrate specificity, making it a key player in cellular signaling pathways.
Research on PP2A B55 and its regulatory subunits is crucial for understanding cell signaling mechanisms and developing targeted therapeutics for diseases like cancer and neurodegenerative disorders.This monoclonal antibody provides researchers with a reliable tool for investigating the function and regulation of PP2A B55 in physiological and pathological conditions. Its high specificity and sensitivity make it a valuable asset for studies in molecular biology, cell biology, and drug discovery. By targeting the PP2A B55 subunit, this antibody opens up new avenues for exploring the role of PP2A in health and disease, offering insights that may lead to novel therapeutic interventions.
Product Name:
PP2A-B55α/PR55α/PPP2R2A Monoclonal Antibody
SKU:
CAB24261
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 94-194 of human PP2A-B55α/PR55α/PPP2R2A (NP_002708.1).
Sequence:
EKIN KIRW LPQK NAAQ FLLS TNDK TIKL WKIS ERDK RPEG YNLK EEDG RYRD PTTV TTLR VPVF RPMD LMVE ASPR RIFA NAHT YHIN SISI NSDY ETYL S
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5520
Clone Number:
ARC60262
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using PP2A-B55α/PR55α/PPP2R2A Rabbit mAb (CAB24261) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
