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PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00428
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Biology
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€599
Frequently bought together:

Description

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PP2A-alpha (Phospho-Tyr307)Colorimetric Cell-Based ELISA Kit

The PP2A Alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit is a cutting-edge assay designed for the precise measurement of PP2A Alpha phosphorylation at the Tyr307 site in cell lysates. This kit boasts unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PP2A Alpha is a critical enzyme involved in cell signaling pathways and cellular processes. Phosphorylation of the Tyr307 site is known to regulate the activity of PP2A Alpha, impacting cell growth, proliferation, and apoptosis. Dysregulation of PP2A Alpha phosphorylation has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, highlighting its importance as a potential therapeutic target.

The PP2A Alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit provides researchers with a valuable tool for studying the role of PP2A Alpha phosphorylation in disease pathogenesis and for developing novel treatments targeting this key regulatory pathway. With its advanced technology and reliable performance, this kit is a must-have for any laboratory conducting cellular signaling research.

Product Name:PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA
Product Code:CBCAB00428
ELISA Type:Cell-Based
Target:PP2A-alpha (Phospho-Tyr307)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PP2A-alpha protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated PP2A-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PP2A-alpha phosphorylation.

Qualitative determination of PP2A-alpha (Phospho-Tyr307) concentration is achieved by an indirect ELISA format. In essence, PP2A-alpha (Phospho-Tyr307) is captured by PP2A-alpha (Phospho-Tyr307)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 5515, UniProt ID: P67775, OMIM: 176915, Unigene: Hs.483408
Gene Symbol:PPP2CA
Sub Type:Phospho
UniProt Protein Function:PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
UniProt Protein Details:

Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function:protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process

NCBI Summary:This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
UniProt Code:P67775
NCBI GenInfo Identifier:54038809
NCBI Gene ID:5515
NCBI Accession:P67775.1
UniProt Related Accession:P67775
Molecular Weight:
NCBI Full Name:Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
NCBI Synonym Full Names:protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol:PPP2CA  
NCBI Official Synonym Symbols:RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha  
NCBI Protein Information:serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Synonym Protein Names:Replication protein C
Protein Family:PP2A regulatory
UniProt Gene Name:PPP2CA  
UniProt Entry Name:PP2AA_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-PP2A-alpha (Phospho-Tyr307) Antibody, Anti-PP2A-alpha Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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