The PP2A A (PR65) Polyclonal Antibody (CAB24836) is a valuable tool for researchers studying the protein phosphatase 2A (PP2A) complex, specifically the regulatory subunit A (PR65/PPP2R1B). This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds to the PR65 subunit of PP2A, allowing for the detection and analysis of this critical protein in various cell types.PP2A is a key phosphatase involved in the regulation of cellular processes, including cell growth, division, and signaling. The PR65 subunit is essential for the structure and function of the PP2A holoenzyme, making it a critical target for research in the fields of cell biology, cancer biology, and drug development.
Understanding the role of the PP2A A subunit in these processes is crucial for advancing our knowledge of disease mechanisms and identifying therapeutic targets.Overall, the PP2A A (PR65) Polyclonal Antibody (CAB24836) is a reliable tool for researchers investigating the PP2A complex and its regulatory subunits. Its specificity and sensitivity make it ideal for various applications in molecular and cellular biology research.
Product Name:
PP2A-Aβ/PR65β/PPP2R1B Polyclonal Antibody
SKU:
CAB24836
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 502-601 of human PP2A-Aβ/PR65β/PPP2R1B(NP_002707.3).
PPP2R1B; PP2A-Abeta; PR65B; protein phosphatase 2 scaffold subunit Abeta; PP2A-Aβ/PR65β/PPP2R1B
Positive Sample:
Rat spleen
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm
Calculated MW:
52kDa/61kDa/66kDa/73kDa
Observed MW:
66kDa
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5519
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat spleen using PP2A-Aβ/PR65β/PPP2R1B Rabbit pAb(CAB24836) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
