The Pou5f1 Polyclonal Antibody (CAB7920) is a valuable tool for researchers studying the transcription factor Pou5f1, also known as Oct4, which plays a key role in pluripotency and stem cell self-renewal. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting and immunohistochemistry applications.Pou5f1/Oct4 is essential for maintaining the pluripotent state of embryonic stem cells and is also involved in the reprogramming of somatic cells to induced pluripotent stem cells. The Pou5f1 Polyclonal Antibody binds specifically to Pou5f1 protein, allowing for accurate detection and analysis in a variety of cell types.
Its high specificity and sensitivity make it an ideal choice for research in stem cell biology, developmental biology, and regenerative medicine.Understanding the mechanisms by which Pou5f1/Oct4 functions is crucial for advancing our knowledge of stem cell biology and potential therapeutic applications. The Pou5f1 Polyclonal Antibody is a valuable tool for investigating the role of Pou5f1 in embryonic development, tissue regeneration, and disease modeling, offering researchers new insights into the complex regulatory networks that govern pluripotency.
Product Name:
OCT4 Rabbit Polyclonal Antibody
SKU:
CAB7920
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 210-310 of human 4-oct (NP_002692.2).
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Purification Method:
Affinity purification
Gene ID:
5460
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse embryo, using OCT4 Rabbit pAb (CAB7920) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
