The POU2F3 Polyclonal Antibody (CAB10054) is a valuable tool for researchers studying the POU2F3 protein, a transcription factor involved in the development of intestinal epithelial cells and regulation of gene expression. This antibody, generated in rabbits, has high specificity for human samples and is validated for use in Western blot applications.POU2F3, also known as Skn-1a, plays a critical role in the differentiation and function of intestinal epithelial cells, making it a key player in maintaining gut homeostasis. Dysregulation of POU2F3 has been implicated in various gastrointestinal disorders, making it a promising target for therapeutic intervention.
By utilizing the POU2F3 Polyclonal Antibody, researchers can effectively detect and analyze POU2F3 protein levels in various cell types, shedding light on its role in intestinal development and function. This antibody is essential for studies in gastrointestinal biology, stem cell research, and digestive disorders, ultimately contributing to the advancement of therapeutic strategies for gastrointestinal diseases.
Product Name:
POU2F3 Rabbit Polyclonal Antibody
SKU:
CAB10054
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human POU2F3 (NP_055167.2).
This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
25833
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using POU2F3 Rabbit pAb (CAB10054) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
