The POU2F1 Polyclonal Antibody (CAB1682) is specially designed for research involving POU2F1, a transcription factor that plays a key role in regulating gene expression. This antibody, generated in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples. By binding to the POU2F1 protein, this antibody enables precise detection and analysis in diverse cell types, making it an excellent tool for studies in molecular biology and developmental biology.POU2F1, also known as OCT1, is a critical transcription factor that regulates the expression of genes involved in cell proliferation, differentiation, and development.
Its importance in gene regulation makes it a valuable target for research into various biological processes, including embryonic development, cell fate determination, and cancer progression. Understanding the function of POU2F1 is essential for unraveling the complex mechanisms underlying these biological processes and for developing targeted therapies that modulate gene expression in disease states.
Product Name:
POU2F1/OCT1 Rabbit Polyclonal Antibody
SKU:
CAB1682
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 15-290 of human POU2F1/OCT1 (NP_001185712.1).
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.
Purification Method:
Affinity purification
Gene ID:
5451
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from rat kidney, using POU2F1/OCT1 Rabbit pAb (CAB1682) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
