The POU1F1 Polyclonal Antibody (CAB17976) is a valuable tool for researchers studying the POU1F1 protein, a key transcription factor involved in pituitary development and hormone regulation. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to POU1F1, it allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in endocrinology and developmental biology.
POU1F1, also known as Pit-1, plays a crucial role in the differentiation and function of hormone-secreting cells in the anterior pituitary gland. Dysregulation of POU1F1 has been linked to various endocrine disorders, making it a promising target for research on hormone-related diseases. Understanding the function and activity of POU1F1 is essential for advancing knowledge of pituitary function and developing potential therapies for hormone imbalances and pituitary tumors.
Product Name:
POU1F1 Rabbit Polyclonal Antibody
SKU:
CAB17976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 124-273 of human POU1F1 (NP_000297.1?).
This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5449
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with POU1F1 using POU1F1 Rabbit pAb (CAB17976) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
