The PORCN Polyclonal Antibody (CAB12250) is a valuable tool for researchers studying PORCN, a protein involved in the Wnt signaling pathway. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the PORCN protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for studies in developmental biology, cancer research, and regenerative medicine.PORCN is a critical component of the Wnt signaling pathway, which plays a key role in various cellular processes such as cell proliferation, differentiation, and migration. Dysregulation of this pathway has been implicated in a wide range of diseases, including cancer and developmental disorders.
By investigating the function of PORCN, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for disease intervention.Furthermore, the availability of a reliable antibody like CAB12250 allows for precise and accurate detection of PORCN expression levels in experimental samples, facilitating experimental reproducibility and enhancing the overall quality of research outcomes. Whether you are investigating the role of PORCN in embryonic development or exploring its implications in cancer progression, this polyclonal antibody is an indispensable tool for advancing your scientific inquiries.
Product Name:
PORCN Rabbit Polyclonal Antibody
SKU:
CAB12250
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1).
This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
64840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PORCN Rabbit pAb (CAB12250) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
