The PORCN Polyclonal Antibody (CAB12120) is a critical tool for researchers studying the PORCN protein, a key regulator of the Wnt signaling pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to PORCN, this antibody enables the detection and analysis of PORCN protein levels in a variety of cell types.PORCN is essential for the acylation and secretion of Wnt proteins, which play a crucial role in embryonic development, tissue homeostasis, and stem cell maintenance. Dysregulation of the Wnt pathway, often involving PORCN, is associated with various diseases, including cancer and developmental disorders.
As such, studying PORCN function with this antibody is vital for uncovering its role in disease pathogenesis and identifying potential therapeutic targets.Researchers in the fields of developmental biology, cancer biology, and regenerative medicine will benefit from the use of the PORCN Polyclonal Antibody in their investigations. Its high specificity and sensitivity make it an indispensable tool for elucidating the complex mechanisms controlled by PORCN and Wnt signaling, ultimately leading to a better understanding of disease processes and the development of targeted treatments.
Product Name:
PORCN Rabbit Polyclonal Antibody
SKU:
CAB12120
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1).
This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
64840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PORCN Rabbit pAb (CAB12120) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
