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Porcine Tumor Necrosis Factor Beta (TNFb) ELISA Kit

SKU:
PRDL00152
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26445
Sensitivity:
5.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
LT,LTA,TNF-B,TNFSF1,Tumor Necrosis Factor Ligand Superfamily Member 1,Lymphotoxin Alpha
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Porcine Tumor Necrosis Factor Beta (TNFb) ELISA Kit

The Porcine Tumor Necrosis Factor Beta (TNFb) ELISA Kit is specifically designed for the accurate measurement of TNFb levels in porcine serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.TNFb is a key cytokine involved in inflammatory and immune responses, playing a crucial role in various physiological and pathological processes.

Its dysregulation has been implicated in conditions such as autoimmune diseases, infectious diseases, and cancer. Therefore, the Porcine TNFb ELISA Kit is an essential tool for studying the role of TNFb in porcine health and disease, as well as for potential therapeutic interventions.

Product Name:Porcine Tumor Necrosis Factor Beta (TNFb) ELISA Kit
Product Code:PRDL00152
Size:96 Assays
Target:TNFb
Synonyms:LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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