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Porcine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

SKU:
PRDL00144
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
K7GSJ9
Sensitivity:
5.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B3,LAP,Latency-associated peptide
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Porcine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

The Porcine Transforming Growth Factor Beta 3 (TGFB3) ELISA Kit is a powerful tool for measuring TGFB3 levels in porcine samples, including serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TGFB3 is a key regulator of cell growth, differentiation, and development, playing a critical role in various physiological processes such as embryogenesis, tissue regeneration, and immune response.

Dysregulation of TGFB3 has been implicated in various diseases, including fibrosis, cancer, and autoimmune disorders, making it a valuable biomarker for studying disease mechanisms and developing targeted therapies.With its reliable performance and versatility, the Porcine TGFB3 ELISA Kit is an essential tool for researchers investigating the role of TGFB3 in health and disease. Get your kit today and unlock new insights into the complex mechanisms of TGFB3 signaling.

Product Name:Porcine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
Product Code:PRDL00144
Size:96 Assays
Target:TGFb3
Synonyms:TGF-B3, LAP, Latency-associated peptide
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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