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Porcine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU:
PRDL00143
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09858
Sensitivity:
10.8pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B2,TGF-Beta2,G-TSF,LAP,Cetermin,Polyergin,Latency-associated peptide,BSC-1 cell growth inhibitor,Glioblastoma-derived T-cell suppressor factor
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Porcine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

The Porcine Transforming Growth Factor Beta 2 (TGFB2) ELISA Kit is specifically designed for the accurate measurement of TGFB2 levels in porcine serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.TGFB2 is a key protein involved in cellular growth, differentiation, and immune responses. It plays a crucial role in various biological processes, including tissue repair, immune regulation, and embryonic development.

Dysregulation of TGFB2 has been linked to various diseases, such as fibrosis, cancer, and inflammatory disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapy development.Overall, the Porcine TGFB2 ELISA Kit is a valuable tool for researchers studying the role of TGFB2 in physiological and pathological processes, providing accurate and precise quantification of this important protein in porcine samples.

Product Name:Porcine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Product Code:PRDL00143
Size:96 Assays
Target:TGFb2
Synonyms:TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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