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Porcine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU:
PRDL00142
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07200
Sensitivity:
10.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B1,CED,DPD1,LAP,Camurati-Engelmann Disease,Latency-associated peptide
Reactivity:
Porcine
Sample Type:
Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Porcine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The Porcine Transforming Growth Factor Beta-1 (TGFB1) ELISA Kit is specifically designed for the precise measurement of TGFB1 levels in porcine serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TGFB1 is a key cytokine involved in controlling cell growth, proliferation, and differentiation, playing a critical role in various biological processes such as embryonic development, immune response, and wound healing.

Dysregulation of TGFB1 has been linked to a range of diseases including cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Porcine TGFB1 ELISA Kit offers researchers a reliable tool for quantifying TGFB1 levels in porcine samples, enabling in-depth investigation into the role of this important cytokine in health and disease.

Product Name:Porcine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit
Product Code:PRDL00142
Size:96 Assays
Target:TGFb1
Synonyms:TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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