The Porcine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is a specialized tool designed for the precise and quantitative detection of Tumor Necrosis Factor Alpha (TNF-α) levels in various biological samples obtained from porcine models. TNF-α is a pivotal pro-inflammatory cytokine that plays a crucial role in modulating cellular processes such as cell proliferation, differentiation, and programmed cell death. It serves as a key mediator in the body's immune response by regulating inflammatory reactions and has been associated with various inflammatory conditions, autoimmune disorders, and infections. Accurate measurement of TNF-α is essential for comprehending its involvement in disease mechanisms and for developing targeted therapeutic interventions.
The Porcine TNF-α ELISA Kit offered by Assay Genie provides exceptional sensitivity and specificity, ensuring dependable and reproducible results. With manufacturing processes adhering to stringent quality control standards, this ELISA kit delivers robust performance that researchers can rely on for their investigations. Its user-friendly nature facilitates ease of use, making it a preferred choice for researchers engaged in porcine immunological studies and inflammatory research, allowing for comprehensive analysis of TNF-α dynamics and immune responses in porcine systems.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine TNF-α. You can calculate the concentration of Porcine TNF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
91-106
98-113
Average (%)
100
98
104
1:4
Range (%)
91-102
80-92
84-95
Average (%)
96
85
90
1:8
Range (%)
93-107
85-99
89-100
Average (%)
99
90
94
1:16
Range (%)
93-108
80-92
83-96
Average (%)
100
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.68
184.99
768.41
87.57
181.59
820.0
Standard deviation
5.57
9.23
27.43
4.52
7.43
38.54
C V (%)
6.01
4.99
3.57
5.16
4.09
4.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine TNF-α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine TNF-α in samples. No significant cross-reactivity or interference between Porcine TNF-α and analogues was observed.
