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Porcine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

SKU:
PRDL00146
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35624
Sensitivity:
13.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
EPO,CLGI,EPA,HCI,Erythroid Potentiating Activity,Collagenase Inhibitor,Metalloproteinase inhibitor 1,Fibroblast collagenase inhibitor
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Porcine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

The Porcine TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) ELISA Kit is a powerful tool for researchers looking to quantify TIMP-1 levels in porcine tissue samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of experimental settings.TIMP-1 is a key regulator of matrix metalloproteinases (MMPs) and plays a critical role in tissue remodeling and repair processes. Dysregulation of TIMP-1 levels has been linked to various diseases, including cancer, arthritis, and cardiovascular diseases, making it an important target for research and potential therapeutic interventions.

With the Porcine TIMP-1 ELISA Kit, researchers can easily assess TIMP-1 levels in porcine samples, providing valuable insights into the role of TIMP-1 in various pathological conditions. This kit is a valuable tool for any laboratory studying tissue remodeling, inflammation, or disease progression in porcine models.

Product Name:Porcine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit
Product Code:PRDL00146
Size:96 Assays
Target:TIMP1
Synonyms:EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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