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Porcine Thrombomodulin (TM) ELISA Kit

SKU:
PRDL00150
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B3STX8
Sensitivity:
0.29ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD141,THBD,BDCA3,THRM,Fetomodulin
Reactivity:
Porcine
Sample Type:
Plasma
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Thrombomodulin (TM) ELISA Kit

The Porcine Thrombomodulin (TM) ELISA Kit is specially designed for the precise detection of thrombomodulin levels in porcine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.Thrombomodulin is a key protein involved in regulating blood coagulation and inflammation processes in the body.

It plays a crucial role in maintaining vascular integrity and preventing thrombosis, making it a valuable biomarker for studying cardiovascular diseases, sepsis, and other related disorders. The Porcine Thrombomodulin ELISA Kit provides researchers with a reliable tool for investigating the roles and functions of thrombomodulin in various physiological and pathological conditions.

Product Name:Porcine Thrombomodulin (TM) ELISA Kit
Product Code:PRDL00150
Size:96 Assays
Target:TM
Synonyms:CD141, THBD, BDCA3, THRM, Fetomodulin
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)90-10095
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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