Porcine TAT (Thrombin/Antithrombin Complex) ELISA Kit
The Porcine TAT (Thrombin/Antithrombin Complex) ELISA Kit is a specialized assay designed for the quantitative detection of TAT levels in porcine samples. The Thrombin/Antithrombin complex is an important marker of coagulation and fibrinolysis processes. Elevated levels of TAT can indicate hypercoagulability in pigs, making this ELISA kit a valuable tool for studying thrombotic disorders, disseminated intravascular coagulation (DIC), and other coagulation-related conditions in porcine models.
Our Porcine TAT ELISA Kit offers exceptional sensitivity and specificity, enabling precise and reproducible measurement of the Thrombin/Antithrombin complex in porcine samples. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, providing researchers with reliable data for studying coagulation pathways and related disorders in porcine research models.
Product Name:
Porcine TAT (Thrombin/Antithrombin Complex) ELISA Kit
SKU:
AEES00282
Target:
Porcine TAT (Thrombin/Antithrombin Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine TAT. You can calculate the concentration of Porcine TAT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
94-109
88-101
Average (%)
100
100
95
1:4
Range (%)
96-108
94-105
86-98
Average (%)
103
99
93
1:8
Range (%)
92-106
96-112
90-102
Average (%)
97
102
97
1:16
Range (%)
94-107
92-108
86-102
Average (%)
101
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
263.37
772.18
2492.03
285.96
801.7
2380.28
Standard deviation
17.49
40.62
112.14
19.79
46.02
112.59
C V (%)
6.64
5.26
4.5
6.92
5.74
4.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine TAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine TAT in samples. No significant cross-reactivity or interference between Porcine TAT and analogues was observed.
