Porcine S100B (S100 Calcium Binding Protein B) ELISA Kit
The Porcine S100B (S100 Calcium Binding Protein B) ELISA Kit is expertly developed to quantitatively detect S100B protein levels in diverse biological samples obtained from porcine subjects. S100B, a member of the S100 family of calcium-binding proteins predominantly expressed in astrocytes, plays a crucial role in various neurological processes, including cell proliferation, differentiation, and neuronal survival. Elevated levels of S100B have been associated with glial cell activation, neuroinflammatory responses, and neurodegenerative conditions, making it a valuable biomarker for studying neurological disorders and brain injuries in porcine models.
With a focus on accuracy and reliability, the Porcine S100B ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, enabling researchers to obtain precise and reproducible results. Manufactured under stringent quality control standards, this ELISA kit provides robust performance, delivering consistent and user-friendly operation for researchers investigating the biological functions of S100B in porcine samples.
Product Name:
Porcine S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
AEES00277
Target:
Porcine S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine S100B. You can calculate the concentration of Porcine S100B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
95-106
93-105
Average (%)
96
101
98
1:4
Range (%)
93-107
83-92
88-100
Average (%)
98
87
94
1:8
Range (%)
91-105
81-93
81-92
Average (%)
96
87
88
1:16
Range (%)
92-103
80-89
83-98
Average (%)
97
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
89-103
94
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.21
4.51
0.44
1.09
4.86
Standard deviation
0.03
0.05
0.2
0.03
0.05
0.17
C V (%)
6.38
4.13
4.43
6.82
4.59
3.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine S100B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine S100B in samples. No significant cross-reactivity or interference between Porcine S100B and analogues was observed.
