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Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

SKU:
PRDL00134
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P80310
Sensitivity:
0.043ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CGRP,P6,CAAF1,CAGC,ENRAGE,MRP6,Calgranulin C,Calcium-binding protein in amniotic fluid 1,Extracellular newly identified RAGE-binding,Migration inhibitory factor-related 6
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

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Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

The Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit is a powerful tool for the accurate measurement of S100A12 levels in porcine samples such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.S100A12 is a calcium-binding protein that plays a critical role in inflammatory processes and immune regulation. Elevated levels of S100A12 have been linked to various inflammatory conditions such as arthritis, inflammatory bowel disease, and sepsis, making it a valuable biomarker for studying these disorders and developing targeted therapies.

With this advanced ELISA kit, researchers can confidently assess S100A12 levels in porcine samples, gaining valuable insights into the role of this important protein in inflammation and disease pathogenesis. Trust in the Porcine S100A12 ELISA Kit for accurate and precise measurement of S100A12 levels in your research studies.

Product Name:Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit
Product Code:PRDL00134
Size:96 Assays
Target:S100A12
Synonyms:CGRP, P6, CAAF1, CAGC, ENRAGE, MRP6, Calgranulin C, Calcium-binding protein in amniotic fluid 1, Extracellular newly identified RAGE-binding, Migration inhibitory factor-related 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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