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Porcine Protransforming growth factor alpha (TGFA) ELISA Kit (PREB0050)

SKU:
PREB0050
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06922
ELISA Type:
Sandwich
Synonyms:
Transforming Growth Factor alpha
Reactivity:
Porcine
$779
Frequently bought together:

Description

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Porcine Protransforming growth factor alpha (TGFA) ELISA Kit

The Porcine Protransforming Growth Factor Alpha (TGFA) ELISA Kit is a powerful tool for the quantitative detection of TGFA levels in porcine samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.TGFA is a key growth factor involved in cell growth, differentiation, and development. Its dysregulation has been linked to various diseases, including cancer, inflammatory disorders, and developmental abnormalities.

By measuring TGFA levels, researchers can gain valuable insights into disease mechanisms and progression, as well as potential therapeutic targets.Overall, the Porcine TGFA ELISA Kit from Assay Genie provides researchers with a precise and efficient tool for studying the role of TGFA in various physiological and pathological processes in porcine models.

Product Name:Porcine Protransforming growth factor alpha (TGFA) ELISA Kit
SKU:PREB0050
Size:96T
Target:Porcine Protransforming growth factor alpha (TGFA)
Synonyms:Protransforming growth factor alpha, TGFA
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Pig
Detection Range:31.2-2000pg/mL
Sensitivity:15.6pg/mL
Intra CV:Provided with the Kit
Inter CV:Provided with the Kit
Linearity:
Sample1:21:41:81:16
Serum(N=5)98-108%95-105%103-112%106-118%
EDTA Plasma(N=5)111-121%90-99%108-118%109-118%
Heparin Plasma(N=5)103-113%87-100%84-93%101-109%
Recovery:Provided with the Kit
Function:TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Uniprot:Q06922
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant pig Protransforming growth factor alpha
Sub Unit:Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.
Research Area:Cancer
Subcellular Location:Protransforming growth factor alpha Cell membrane Single-pass type I membrane protein
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q06922
NCBI GenInfo Identifier:55741795
NCBI Gene ID:397484
NCBI Accession:NP_999416.1
UniProt Secondary Accession:Q06922
UniProt Related Accession:Q06922
Molecular Weight:17,056 Da
NCBI Full Name:protransforming growth factor alpha
NCBI Synonym Full Names:
NCBI Official Symbol:TGFA
NCBI Official Synonym Symbols:
NCBI Protein Information:protransforming growth factor alpha
UniProt Protein Name:Protransforming growth factor alpha
UniProt Synonym Protein Names:EGF-like TGF; ETGF; TGF type 1
Protein Family:Transforming growth factor
UniProt Gene Name:TGFA
UniProt Entry Name:TGFA_PIG
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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