The Porcine PP (Pepsin) ELISA Kit is a specialized assay crafted for the precise measurement of Pepsin levels in porcine biological samples. Pepsin, an essential enzyme in gastric physiology, plays a crucial role in the breakdown of proteins into peptides during digestion, particularly in the stomach of pigs. Monitoring Pepsin concentrations enables researchers to assess gastric function, digestive health, and nutrient absorption in porcine models. This ELISA kit offers a reliable and accurate method for quantifying Pepsin levels, providing valuable insights into porcine digestive processes and gastric health.
By measuring Pepsin concentrations, researchers can evaluate the enzymatic activity in the stomach, investigate digestive disorders, and explore dietary influences on the digestive system of pigs. With exceptional sensitivity and specificity, the Porcine PP ELISA Kit ensures precise and reproducible results, facilitating in-depth studies on porcine gastrointestinal function and nutritional metabolism. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an indispensable tool for research aimed at understanding porcine digestive physiology and dietary impact.
Product Name:
Porcine PP (Pepsin) ELISA Kit
SKU:
AEES00691
Target:
Porcine PP (Pepsin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
46.88 ng/mL
Detection range:
78.13-5000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine PP. You can calculate the concentration of Porcine PP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
86-96
93-107
Average (%)
99
91
99
1:4
Range (%)
92-105
80-91
90-106
Average (%)
99
87
97
1:8
Range (%)
95-109
83-94
92-103
Average (%)
100
88
98
1:16
Range (%)
92-105
80-93
92-106
Average (%)
99
87
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
255.72
685.23
2383.72
248.85
620.38
2430.71
Standard deviation
17.67
33.1
76.76
16.05
33.75
98.69
C V (%)
6.91
4.83
3.22
6.45
5.44
4.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine PP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine PP in samples. No significant cross-reactivity or interference between Porcine PP and analogues was observed.
