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Porcine PD-1/PDCD1(Programmed Cell Death Protein 1) ELISA Kit

SKU:
AEFI00805
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
18.75pg/ml
Range:
31.2-2000pg/ml
ELISA Type:
Sandwich
Reactivity:
Porcine
$779
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Description

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Porcine PD-1/PDCD1 (Programmed Cell Death Protein 1) ELISA Kit

The Porcine PD-1 (PDCD1) Programmed Cell Death Protein 1 ELISA Kit is a highly sensitive and reliable tool for the accurate detection of PD-1 levels in porcine samples. This kit is designed for use with porcine serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results.PD-1 is a key immune checkpoint molecule that plays a crucial role in regulating immune responses and maintaining immune tolerance. Dysregulation of PD-1 expression has been implicated in various diseases, including cancer, autoimmune disorders, and infectious diseases.

Therefore, studying PD-1 levels in porcine models can provide valuable insights into immune function and disease development.By utilizing the Porcine PD-1 ELISA Kit, researchers can effectively measure PD-1 levels in porcine samples, advancing their understanding of immune modulation and immune-related diseases. This kit offers exceptional sensitivity and specificity, making it an essential tool for a wide range of research applications in the fields of immunology, oncology, and infectious diseases.

Product Name:Porcine PD-1/PDCD1(Programmed Cell Death Protein 1) ELISA Kit
Product Code:AEFI00805
Size:96T
Alias:PD-1/PDCD1, Programmed Cell Death Protein 1
Detection method:Sandwich ELISA, Double Antibody
Application:PD-1/PDCD1 ELISA Kit allows for the in vitro quantitative determination of PD-1/PDCD1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:< 18.75pg/ml
Range:31.2-2000pg/ml
Storage:2-8°C for 6 months
Note:For Research Use Only
Recovery: Matrices were spiked with Porcine PD-1/PDCD1(Programmed Cell Death Protein 1) and the recovery rates were calculated by comparing the measured value to the expected amount of Porcine PD-1/PDCD1(Programmed Cell Death Protein 1) in samples. Enquire for more information.
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Porcine PD-1/PDCD1(Programmed Cell Death Protein 1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information.
CV(%): Intra-Assay: CV<8%
Inter-Assay: CV<10%

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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