null

Porcine NPY (Neuropeptide Y) ELISA Kit (AEES00707)

SKU:
AEES00707
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Porcine
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
€499
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Porcine NPY (Neuropeptide Y) ELISA Kit
SKU:AEES00707
Target:Porcine NPY (Neuropeptide Y)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.5h
Sensitivity:18.75 pg/mL
Detection range:31.25-2000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NPY. During the reaction, NPY in the sample or standard competes with a fixed amount of NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NPY. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPY in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)88-10288-10495-109
Average (%)9395103
1:4Range (%)87-10285-9896-110
Average (%)9390103
1:8Range (%)86-9892-10497-112
Average (%)9397102
1:16Range (%)90-10190-10398-115
Average (%)9596105
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)85-9890
EDTA plasma (n=5)85-9892
Cell culture media (n=5)90-10698
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)92.87271.37937.5597.88275.681003.8
Standard deviation5.8912.5142.385.0314.4546.38
C V (%)6.344.614.525.145.244.62
Sample type &Sample volume:; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of NPY concentrations in .
Specificity:This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.

Related Products