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Porcine Nerve Growth Factor (NGF) ELISA Kit

SKU:
PRDL00117
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q29074
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NGFB,Beta-NGF,HSAN5,NGF-B,Beta-Nerve Growth Factor
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Porcine Nerve Growth Factor (NGF) ELISA Kit

The Porcine Nerve Growth Factor (NGF) ELISA Kit is specifically designed for the accurate detection of nerve growth factor levels in porcine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Nerve growth factor is a critical protein that plays a key role in the growth, maintenance, and survival of nerve cells. It is essential for the development and function of the nervous system, making it a valuable biomarker for studying neurological disorders, nerve regeneration, and potential therapeutic interventions.

With this advanced ELISA kit, researchers can confidently measure and analyze porcine NGF levels, providing valuable insights into the mechanisms underlying nerve growth and development. This kit is an indispensable tool for investigating nerve-related diseases and advancing our understanding of neurobiology.

Product Name:Porcine Nerve Growth Factor (NGF) ELISA Kit
Product Code:PRDL00117
Size:96 Assays
Target:NGF
Synonyms:IGF-1,IGF-I,Insulin-like growth factor I,Somatomedin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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