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Porcine Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit

SKU:
PRDL00104
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P42831
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit

The Porcine Monocyte Chemotactic Protein-1 (MCP1) ELISA Kit offered by AssayGenie is a reliable and easy-to-use tool for detecting MCP1 levels in porcine samples. This kit is designed for use with porcine serum, plasma, and cell culture supernatants, providing researchers with accurate and reproducible results.MCP1 is a key chemokine involved in recruiting monocytes to sites of inflammation, making it an important biomarker for studying immune responses and inflammatory conditions in pigs. By accurately measuring MCP1 levels, researchers can better understand the role of this protein in various diseases and develop potential therapeutic strategies.

With its high sensitivity and specificity, the Porcine MCP1 ELISA Kit from AssayGenie is an essential tool for researchers working in the fields of immunology, inflammation, and veterinary medicine. This kit is suitable for a wide range of applications, providing valuable insights into the role of MCP1 in porcine health and disease.

Product Name:Porcine Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
Product Code:PRDL00104
Size:96 Assays
Target:MCP1
Synonyms:CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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