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Porcine Matrix Metalloproteinase 2 (MMP2) ELISA Kit

SKU:
PRDL00110
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q95JA4
Sensitivity:
33pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CLG4,CLG4A,MMP-II,MONA,TBE-1,PEX,Gelatinase A,72kDa Gelatinase,72kDa Type IV Collagenase
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Matrix Metalloproteinase 2 (MMP2) ELISA Kit

The Porcine Matrix Metalloproteinase-2 (MMP-2) ELISA Kit is a reliable and accurate tool for the detection of MMP-2 levels in porcine samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results in serum, plasma, and cell culture supernatants.MMP-2 is a key enzyme involved in the remodeling of the extracellular matrix, playing a crucial role in various physiological and pathological processes such as tissue repair, inflammation, and cancer metastasis.

Monitoring MMP-2 levels can provide valuable insights into the progression of diseases and the efficacy of therapeutic interventions.Whether studying porcine models of diseases or investigating the role of MMP-2 in various biological pathways, the Porcine MMP-2 ELISA Kit is an essential tool for researchers in the field of molecular biology and medicine. Get accurate and reliable results with this advanced kit from AssayGenie.

Product Name:Porcine Matrix Metalloproteinase 2 (MMP2) ELISA Kit
Product Code:PRDL00110
Size:96 Assays
Target:MMP2
Synonyms:CLG4, CLG4A, MMP-II, MONA, TBE-1, PEX, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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