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Porcine Matrix Metalloproteinase 1 (MMP1) ELISA Kit

SKU:
PRDL00108
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21692
Sensitivity:
0.73ng/mL
Range:
2.343-150ng/mL
ELISA Type:
Sandwich
Synonyms:
CLGN,CLG1,Collagenase,Interstitial Collagenase,Vertebrate Collagenase,Fibroblast Collagenase
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Porcine Matrix Metalloproteinase 1 (MMP1) ELISA Kit

The Porcine Matrix Metalloproteinase-1 (MMP1) ELISA Kit from Assay Genie is specifically designed for the accurate quantification of MMP1 levels in porcine samples. MMP1 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in tissue remodeling, wound healing, and various disease processes.This ELISA kit offers high sensitivity and specificity, ensuring precise and reliable results for porcine serum, plasma, and cell culture supernatants.

With its easy-to-use format and quick assay time, this kit is ideal for researchers studying matrix metalloproteinases and their involvement in various physiological and pathological processes in pigs.Overall, the Porcine MMP1 ELISA Kit is a valuable tool for researchers looking to investigate the role of MMP1 in porcine health and disease, providing a comprehensive understanding of its functions and potential implications for therapeutic development.

Product Name:Porcine Matrix Metalloproteinase 1 (MMP1) ELISA Kit
Product Code:PRDL00108
Size:96 Assays
Target:MMP1
Synonyms:CLGN, CLG1, Collagenase, Interstitial Collagenase, Vertebrate Collagenase, Fibroblast Collagenase
Detection Method:Sandwich
Range:2.343-150ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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