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Porcine Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

SKU:
PRDL00107
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D0Z5W4
Sensitivity:
7.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL19,CKb11,ELC,MIP3-B,SCYA19,Chemokine C-C-Motif Ligand 19,Beta Chemokine Exodus-3,CK Beta-11,EBI1-Ligand Chemokine,Small Inducible Cytokine Subfamily A19
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

The Porcine Macrophage Inflammatory Protein 3 Beta (MIP3B) ELISA Kit is specially designed for the accurate measurement of MIP3B levels in porcine serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for research in various fields.MIP3B is a key chemokine involved in the recruitment and activation of macrophages, playing a critical role in the inflammatory response.

It is also implicated in immune system regulation and has been linked to various inflammatory diseases. Therefore, the Porcine MIP3B ELISA Kit is invaluable for studying these conditions and potentially developing targeted therapies.Overall, this kit offers researchers a precise and efficient method for quantifying MIP3B levels in porcine samples, allowing for in-depth investigations into the role of this chemokine in inflammation and immunity.

Product Name:Porcine Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit
Product Code:PRDL00107
Size:96 Assays
Target:MIP3b
Synonyms:CCL19, CKb11, ELC, MIP3-B, SCYA19, Chemokine C-C-Motif Ligand 19, Beta Chemokine Exodus-3, CK Beta-11, EBI1-Ligand Chemokine, Small Inducible Cytokine Subfamily A19
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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