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Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU:
PRDL00106
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q711P4
Sensitivity:
12.7pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL4,ACT2,G-26,LAG1,MIP1-B,SCYA4,Chemokine C-C-Motif Ligand 4,Small Inducible Cytokine A4,Homologous To Mouse Mip-1b
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

The Porcine Macrophage Inflammatory Protein-1 Beta (MIP-1B) ELISA kit from Assay Genie is specially designed for the accurate detection of MIP-1B levels in porcine serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for a variety of research applications.MIP-1B is a key chemokine involved in the immune response, playing a critical role in inflammation and immune cell recruitment.

It is essential for understanding and studying various inflammatory conditions and immune responses in porcine models. The Assay Genie Porcine MIP-1B ELISA kit provides researchers with a valuable tool for investigating the role of MIP-1B in porcine health and disease, enabling the development of potential therapeutic strategies.

Product Name:Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Product Code:PRDL00106
Size:96 Assays
Target:MIP1b
Synonyms:CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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