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Porcine Lipopolysaccharide Binding Protein (LBP) ELISA Kit

SKU:
PRDL00098
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B3F714
Sensitivity:
35ng/mL
Range:
78.12-5000ng/mL
ELISA Type:
Sandwich
Synonyms:
LPS-Binding Protein
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
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Description

system_update_altDatasheet

Porcine Lipopolysaccharide Binding Protein (LBP) ELISA Kit

The Porcine Lipopolysaccharide Binding Protein (LBP) ELISA Kit is specifically designed for the accurate measurement of porcine LBP levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.LBP is a key protein involved in the immune response to Gram-negative bacterial infections, specifically in the recognition and binding of lipopolysaccharides (LPS), an important component of the bacterial cell wall.

Monitoring LBP levels can provide valuable insights into the host immune response and the development of infectious diseases in pigs.This ELISA kit is essential for researchers studying the immune system of pigs, infectious diseases, and inflammatory conditions. With its advanced technology and easy-to-use protocol, the Porcine LBP ELISA Kit is a valuable tool for accurately measuring LBP levels in porcine samples.

Product Name:Porcine Lipopolysaccharide Binding Protein (LBP) ELISA Kit
Product Code:PRDL00098
Size:96 Assays
Target:LBP
Synonyms:LPS-Binding Protein
Detection Method:Sandwich
Range:78.1-5,000ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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