The Porcine LH (Luteinizing Hormone) ELISA Kit is meticulously designed to quantitatively detect luteinizing hormone levels in various porcine biological samples. Luteinizing hormone, a pivotal glycoprotein hormone secreted by the anterior pituitary gland, plays a vital role in reproductive functions, specifically in the regulation of ovulation and sex steroid hormone production. By accurately measuring LH levels, researchers can gain insights into the porcine reproductive cycle, ovulation timing, and reproductive health, making this ELISA kit an invaluable tool for studies related to porcine reproductive physiology and endocrinology.
Assay Genie's Porcine LH ELISA Kit offers unparalleled sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit guarantees robust performance and ease of use, providing researchers with a reliable and efficient method for investigating luteinizing hormone dynamics in porcine samples.
Product Name:
Porcine LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00278
Target:
Porcine LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine LH. You can calculate the concentration of Porcine LH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
95-110
85-98
Average (%)
100
102
90
1:4
Range (%)
88-101
81-93
87-100
Average (%)
93
86
94
1:8
Range (%)
89-103
83-97
85-98
Average (%)
96
89
92
1:16
Range (%)
90-102
82-98
85-96
Average (%)
95
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.56
6.37
20.33
2.81
6.33
18.63
Standard deviation
0.13
0.37
1.01
0.19
0.34
0.95
C V (%)
5.08
5.81
4.97
6.76
5.37
5.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine LH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine LH in samples. No significant cross-reactivity or interference between Porcine LH and analogues was observed.
