Porcine ITIH4 (Inter-alpha-trypsin Inhibitor Heavy Chain H4) ELISA Kit
The Porcine ITIH4 (Inter-alpha-trypsin Inhibitor Heavy Chain H4) ELISA Kit is a specialized assay designed for the precise quantitative measurement of Inter-alpha-trypsin inhibitor heavy chain H4 levels in porcine biological samples. ITIH4 is a key protease inhibitor involved in modulating inflammation and extracellular matrix stability, playing a crucial role in various biological processes, including regulation of protease activity and immune response modulation. This ELISA kit provides researchers with a reliable tool to study the levels of ITIH4 in porcine samples, shedding light on its role in inflammation, tissue repair, and overall health in pigs.
Inter-alpha-trypsin inhibitor heavy chain H4 is essential in maintaining tissue homeostasis and is associated with inflammatory responses and disease pathogenesis. With exceptional sensitivity and specificity, the Porcine ITIH4 ELISA Kit ensures accurate and reproducible results, enabling researchers to investigate the intricate functions of ITIH4 in porcine physiology. Manufactured under stringent quality control standards, this kit offers robust performance and ease of use, making it an excellent choice for research studies focusing on protease regulation and inflammatory processes in pigs.
Product Name:
Porcine ITIH4 (Inter-alpha-trypsin Inhibitor Heavy Chain H4) ELISA Kit
SKU:
AEES00709
Target:
Porcine ITIH4 (Inter-alpha-trypsin Inhibitor Heavy Chain H4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine ITIH4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine ITIH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine ITIH4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine ITIH4. You can calculate the concentration of Porcine ITIH4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
88-99
87-100
Average (%)
103
94
93
1:4
Range (%)
93-109
86-101
89-103
Average (%)
99
92
96
1:8
Range (%)
94-110
87-99
88-99
Average (%)
101
92
94
1:16
Range (%)
96-112
86-96
86-96
Average (%)
103
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
98
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
94-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.21
25.05
98.62
9.59
25.99
97.11
Standard deviation
0.59
1.15
4.31
0.51
1.19
3.19
C V (%)
6.41
4.59
4.37
5.32
4.58
3.28
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine ITIH4 concentrations in .
Specificity:
This kit recognizes Porcine ITIH4 in samples. No significant cross-reactivity or interference between Porcine ITIH4 and analogues was observed.
