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Porcine Interleukin 6 (IL-6) ELISA Kit

SKU:
PRDL00093
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26893
Sensitivity:
26.6pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
MGI2-A,MGI2A,HGF,BSF2,HSF,IFNB2,B-Cell Stimulatory Factor-2,Hybridoma/Plasmacytoma Growth Factor,Hepatocyte Stimulating Factor,Cytotoxic T-Cell Differentiation Factor
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Porcine Interleukin 6 (IL-6) ELISA Kit

The Porcine Interleukin-6 (IL-6) ELISA Kit offered by Assay Genie is a cutting-edge tool designed for the precise measurement of IL-6 levels in porcine serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide array of research purposes.IL-6 is a key cytokine involved in immune response and inflammation, with implications in various physiological and pathological processes. It serves as a vital marker in studying conditions such as infectious diseases, autoimmune disorders, and cancer, thus holding significant value in biomedical research and drug development efforts.

The Assay Genie Porcine IL-6 ELISA Kit provides researchers with a reliable and efficient means to investigate the role of IL-6 in porcine health and disease, facilitating a deeper understanding of its functions and potential therapeutic applications. Trust in the precision and performance of this advanced kit to propel your research forward.

Product Name:Porcine Interleukin 6 (IL-6) ELISA Kit
Product Code:PRDL00093
Size:96 Assays
Target:IL6
Synonyms:MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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