null

Porcine Interleukin 12A (IL-12A) ELISA Kit

SKU:
PRDL00082
Product Type:
ELISA Kit
Sensitivity:
8.42pg/ml
Range:
31.25-2000pg/ml
ELISA Type:
Sandwich
Synonyms:
p35,IL12-A,CLMF1,NFSK,NKSF1,Natural Killer Cell Stimulatory Factor 1,Cytotoxic Lymphocyte Maturation Factor 1,NF Cell Stimulatory Factor Chain 1
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Porcine Interleukin 12A (IL-12A) ELISA Kit

The Porcine Interleukin-12A (IL-12A) ELISA Kit is specifically designed for the precise measurement of IL-12A levels in porcine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.IL-12A is a key cytokine that plays a crucial role in immune responses, particularly in the activation of T cells and natural killer cells. It is involved in the regulation of inflammatory processes and the development of immune-mediated diseases.

Studying IL-12A levels can provide valuable insights into immune system function and potential therapeutic targets for a range of diseases.With its reliable performance and ease of use, the Porcine Interleukin-12A ELISA Kit is an essential tool for researchers looking to investigate the role of IL-12A in immune regulation and disease pathogenesis in porcine models.

Product Name:Porcine Interleukin 12A (IL-12A) ELISA Kit
Product Code:PRDL00082
Size:96 Assays
Target:IL12A
Synonyms:p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products