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Porcine Interleukin 1 Alpha (IL-1a) ELISA Kit

SKU:
PRDL00088
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18430
Sensitivity:
5.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IL1-A,IL-1α,IL1F1,Preinterleukin 1 Alpha,Hematopoietin-1,Pro-Interleukin-1-Alpha,Interleukin-1 Family Member 1
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Porcine Interleukin 1 Alpha (IL-1a) ELISA Kit

The Porcine Interleukin-1 Alpha (IL-1A) ELISA Kit is a powerful tool for accurate detection of IL-1A levels in porcine serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results, perfect for various research applications.IL-1A is a vital cytokine involved in inflammatory responses and immune regulation. It plays a crucial role in various diseases, including infectious diseases, autoimmune disorders, and inflammatory conditions.

Studying IL-1A levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of novel therapeutic strategies.Overall, the Porcine IL-1A ELISA Kit is a reliable and efficient tool for researchers looking to investigate the role of IL-1A in porcine health and disease.

Product Name:Porcine Interleukin 1 Alpha (IL-1a) ELISA Kit
Product Code:PRDL00088
Size:96 Assays
Target:IL1a
Synonyms:IL1-A, IL-1?, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha, Interleukin-1 Family Member 1
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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