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Porcine IL-1β (Interleukin 1 Beta) ELISA Kit

SKU:
AEES00272
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Porcine
Sensitivity:
37.50 pg/mL
Range:
62.50-4000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Porcine IL-1β ELISA Kit

The Porcine IL-1β ELISA Kit is a specialized assay designed for the precise and quantitative measurement of Interleukin-1 beta (IL-1β) levels in a variety of biological samples obtained from porcine models. IL-1β is a vital pro-inflammatory cytokine central to numerous cellular functions, such as cell proliferation, differentiation, and programmed cell death. It serves as a key mediator in the immune system, orchestrating inflammatory responses and has been linked to various inflammatory conditions, autoimmune diseases, and infectious processes. Accurate quantification of IL-1β is essential for elucidating its mechanistic role in disease pathophysiology and for devising targeted intervention strategies.

The Porcine IL-1β ELISA Kit provided by Assay Genie exemplifies exceptional sensitivity and specificity, enabling researchers to generate reliable and reproducible results. Manufactured under stringent quality control guidelines, this ELISA kit ensures robust performance, delivering precise data that researchers can depend on for their investigations. Its user-friendly protocols further simplify experimental workflows, making it an ideal choice for porcine immunology and inflammatory research, offering a comprehensive solution for exploring IL-1β dynamics and immunological responses in porcine models.

Product Name:Porcine IL-1β (Interleukin 1 Beta) ELISA Kit
Product Code:AEES00272
Assay Type:Sandwich
Format:96T
Assay Time:4.5h
Reactivity:Porcine
Detection Range:62.50-4000 pg/mL
Sensitivity:37.50 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Porcine IL-1β in samples. No significant cross-reactivity or interference between Porcine IL-1β and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Porcine IL-1β concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine IL-1β. You can calculate the concentration of Porcine IL-1β in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine IL-1β were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine IL-1β were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)209.37520.891471.35200.05560.841568.14
Standard deviation11.6429.0776.2211.0228.7756.14
CV (%)5.565.585.185.515.133.58

Recovery

The recovery of Porcine IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)90-10798
EDTA plasma (n=8)85-10192
Cell culture media (n=8)84-9991
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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