The Porcine IgG (Immunoglobulin G) ELISA Kit is a specialized assay designed for the precise and quantitative detection of IgG levels in porcine biological samples. Immunoglobulin G, a crucial component of the humoral immune response, plays a fundamental role in host defense mechanisms against pathogens and foreign antigens. As the most abundant antibody isotype in circulation, IgG mediates various immune functions, including pathogen neutralization, opsonization, and immune complex formation. This ELISA kit is designed to accurately quantify IgG concentrations in porcine samples, providing valuable insights into the humoral immune response in swine models.
By measuring IgG levels, researchers can assess the antibody-mediated immune response, monitor immune status, and investigate the role of IgG in host defense and infection control in porcine studies. The Porcine IgG ELISA Kit offers exceptional sensitivity and specificity, enabling reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an ideal tool for investigating immune responses, immunity, and infectious diseases in pigs.
Product Name:
Porcine IgG (Immunoglobulin G) ELISA Kit
SKU:
AEES00705
Target:
Porcine IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine IgG. You can calculate the concentration of Porcine IgG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
96-110
97-112
Average (%)
102
103
105
1:4
Range (%)
93-106
86-98
88-103
Average (%)
100
91
94
1:8
Range (%)
93-107
87-101
89-103
Average (%)
99
92
94
1:16
Range (%)
91-103
80-93
87-98
Average (%)
98
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11.05
25.65
93.47
10.02
23.09
91.68
Standard deviation
0.7
1.21
4.06
0.65
1.13
4.64
C V (%)
6.33
4.72
4.34
6.49
4.89
5.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine IgG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine IgG in samples. No significant cross-reactivity or interference between Porcine IgG and analogues was observed.
