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Porcine Growth Hormone Releasing Hormone (GHRH) ELISA Kit

SKU:
PRDL00059
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01287
Sensitivity:
5.78pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Competitive
Synonyms:
GRF,GHRF,Somatocrinin,Growth-Hormone-Releasing Factor,Sermorelin,Somatoliberin
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Growth Hormone Releasing Hormone (GHRH) ELISA Kit

The Porcine Growth Hormone-Releasing Hormone (GHRH) ELISA Kit is specifically designed for the accurate quantification of porcine GHRH levels in biological samples such as serum, plasma, and tissue lysates. This kit offers superior sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.GHRH is a key regulator of growth hormone release in pigs, influencing growth, metabolism, and reproduction. Understanding the levels of GHRH can provide valuable insights into the physiological processes controlled by this hormone, making it a valuable tool for studying growth-related disorders and developing potential interventions.

With its user-friendly protocol and reliable performance, the Porcine GHRH ELISA Kit is an indispensable tool for researchers and clinicians studying the role of GHRH in porcine physiology and disease. Order now to discover new insights into porcine growth regulation.

Product Name:Porcine Growth Hormone Releasing Hormone (GHRH) ELISA Kit
Product Code:PRDL00059
Size:96 Assays
Target:GHRH
Synonyms:GRF GHRF Somatocrinin Growth-Hormone-Releasing Factor Sermorelin Somatoliberin
Detection Method:Competition
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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