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Porcine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

SKU:
PRDL00050
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F1RQN3
Sensitivity:
41.78pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
B-FGF,BFGF,FGFB,HBGH-2,Basic Fibroblast Growth Factor,Heparin-binding growth factor 2
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Porcine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

The Porcine Fibroblast Growth Factor 2 (FGF2) ELISA Kit is specifically designed for the accurate detection of FGF2 levels in porcine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers working with porcine samples.FGF2 is a key growth factor involved in cell proliferation, differentiation, and tissue repair. It plays a critical role in various biological processes, including wound healing, development, and angiogenesis.

Monitoring FGF2 levels in porcine samples can provide valuable insights into these processes, aiding in the study of various diseases and potential therapeutic interventions.With its advanced technology and user-friendly protocols, the Porcine FGF2 ELISA Kit is a valuable tool for researchers looking to investigate the role of FGF2 in porcine physiology and pathology. Get precise and meaningful results with this reliable assay kit from Assay Genie.

Product Name:Porcine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit
Product Code:PRDL00050
Size:96 Assays
Target:FGF2
Synonyms:B-FGF, BFGF, FGFB, HBGH-2, Basic Fibroblast Growth Factor, Heparin-binding growth factor 2
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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