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Porcine Factor Related Apoptosis (FAS) ELISA Kit

SKU:
PRDL00047
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O77736
Sensitivity:
7.2pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD95,ALPS1A,ALPS1-A,APO1,APT1,FAS1,FASTM,TNFRSF6,Fas Receptor,TNF Receptor Superfamily Member 6,Tumor Necrosis Factor Receptor Superfamily Member 6
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Factor Related Apoptosis (FAS) ELISA Kit

The Porcine Factor-Related Apoptosis (FAS) ELISA Kit from Assay Genie is a cutting-edge tool for researchers looking to accurately measure the levels of FAS in porcine samples. FAS is a key protein involved in apoptosis, or programmed cell death, and plays a critical role in various cellular processes.This ELISA kit boasts high sensitivity and specificity, ensuring precise and reliable results every time. It is suitable for use with porcine serum, plasma, and tissue culture supernatants, making it versatile for a wide range of research applications.

By measuring FAS levels, researchers can gain valuable insights into the mechanisms of cell death and survival, as well as the potential implications for various diseases and conditions. This kit is a valuable asset for those studying apoptosis, cancer, autoimmune disorders, and more. Trust Assay Genie for accurate and efficient FAS detection in porcine samples.

Product Name:Porcine Factor Related Apoptosis (FAS) ELISA Kit
Product Code:PRDL00047
Size:96 Assays
Target:FAS
Synonyms:CD95, ALPS1A, ALPS1-A, APO1, APT1, FAS1, FASTM, TNFRSF6, Fas Receptor, TNF Receptor Superfamily Member 6, Tumor Necrosis Factor Receptor Superfamily Member 6
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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