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Porcine Epidermal Growth Factor (EGF) ELISA Kit

SKU:
PRDL00043
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q00968
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
URG,Beta-Urogastrone,Pro-epidermal growth factor
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Porcine Epidermal Growth Factor (EGF) ELISA Kit

The Porcine Epidermal Growth Factor (EGF) ELISA Kit is a reliable tool for the accurate quantification of EGF levels in porcine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.EGF is a key growth factor known for its role in promoting cell growth, proliferation, and differentiation. It plays a crucial role in wound healing, tissue repair, and cellular regeneration, making it a valuable biomarker for studying these processes and developing therapeutic strategies.

With its user-friendly protocol and robust performance, the Porcine EGF ELISA Kit is an essential tool for researchers studying growth factors, cell signaling pathways, and tissue regeneration mechanisms in porcine models. Trust in this kit to deliver accurate and reliable data for your research needs.

Product Name:Porcine Epidermal Growth Factor (EGF) ELISA Kit
Product Code:PRDL00043
Size:96 Assays
Target:EGF
Synonyms:URG, Beta-Urogastrone, Pro-epidermal growth factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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