The Porcine CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit is a specialized assay designed for the accurate and quantitative measurement of CKMB levels in porcine samples.
CKMB is an enzyme found in heart muscle cells and is a well-established biomarker used in the diagnosis of myocardial infarction, ischemic heart disease, and other cardiac-related conditions in pigs. Monitoring CKMB levels provides valuable information about heart muscle damage and function, making it a crucial tool in cardiac research and diagnostics involving swine models. Our Porcine CKMB ELISA Kit offers superior sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and ease of use, making it an excellent choice for investigating creatine kinase MB in porcine samples for various research applications in veterinary medicine and cardiology.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine CKMB. You can calculate the concentration of Porcine CKMB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
98-111
95-108
Average (%)
91
106
103
1:4
Range (%)
85-95
98-117
93-106
Average (%)
90
106
100
1:8
Range (%)
83-96
100-112
96-108
Average (%)
90
105
102
1:16
Range (%)
87-99
98-110
98-110
Average (%)
93
104
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
99
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.7
1.43
5.56
0.74
1.36
6.01
Standard deviation
0.05
0.08
0.24
0.05
0.07
0.2
C V (%)
7.14
5.59
4.32
6.76
5.15
3.33
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine CKMB concentrations in .
Specificity:
This kit recognizes Porcine CKMB in samples. No significant cross-reactivity or interference between Porcine CKMB and analogues was observed.
