The Porcine C3a (Complement Component 3a) ELISA Kit is a specialized assay designed for the precise quantification of C3a levels in porcine biological samples. C3a, a key component of the complement system, plays a vital role in the regulation of inflammatory responses and immune modulation. As an anaphylatoxin, C3a is involved in various immune processes, including chemotaxis, cell activation, and inflammation, contributing to the body's defense against pathogens and foreign entities. This innovative ELISA kit enables researchers to accurately measure C3a concentrations in porcine samples, facilitating detailed investigations into the complement system's role in immune regulation and inflammatory pathways.
By quantifying C3a levels, researchers can explore the dynamics of complement activation, immune responses, and inflammatory cascades in porcine models, providing valuable insights into immune-mediated processes and disease mechanisms. The Porcine C3a ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and ease of use, making it an excellent choice for porcine studies in immunology, inflammation, and immune response modulation.
Product Name:
Porcine C3a (Complement Component 3a) ELISA Kit
SKU:
AEES00704
Target:
Porcine C3a (Complement Component 3a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine C3a. You can calculate the concentration of Porcine C3a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
91-104
94-107
Average (%)
92
99
100
1:4
Range (%)
87-98
90-100
94-106
Average (%)
93
95
100
1:8
Range (%)
81-92
95-108
91-103
Average (%)
87
100
97
1:16
Range (%)
82-94
88-101
93-107
Average (%)
88
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
97-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.52
5.02
21.76
2.71
5.07
19.82
Standard deviation
0.14
0.25
1.03
0.18
0.24
0.65
C V (%)
5.56
4.98
4.73
6.64
4.73
3.28
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine C3a concentrations in .
Specificity:
This kit recognizes Porcine C3a in samples. No significant cross-reactivity or interference between Porcine C3a and analogues was observed.
